s protein Search Results


omicron spike trimer conjugated  (R&D Systems)
91
R&D Systems omicron spike trimer conjugated
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Omicron Spike Trimer Conjugated, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/Recombinant+SARS-CoV-2+B%2E1%2E1%2E529+S+Alexa+Fluor%C2%AE+488+Protein/pm36848880-104-2-10
Average 91 stars, based on 1 article reviews
omicron spike trimer conjugated - by Bioz Stars, 2026-09
91/100 stars
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water  (Inotiv)
99
Inotiv water
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Water, supplied by Inotiv, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/Teklad+Irradiated+Global+Diet+2918/10__1093_slash_jbmrpl_slash_ziag060-109-27-23
Average 99 stars, based on 1 article reviews
water - by Bioz Stars, 2026-09
99/100 stars
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protein  (ACROBiosystems)
95
ACROBiosystems protein
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Protein, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/SARS-CoV-2+S+protein+(L18F%2C+D80A%2C+D215G%2C+242-244del%2C+R246I%2C+K417N%2C+E484K%2C+N501Y%2C+D614G%2C+A701V)+trimer%2C+His+Tag/pmc12061477-19-6-14
Average 95 stars, based on 1 article reviews
protein - by Bioz Stars, 2026-09
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sars cov 2 spike protein  (ACROBiosystems)
95
ACROBiosystems sars cov 2 spike protein
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Sars Cov 2 Spike Protein, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/SARS-CoV-2+S+protein%2C+His+Tag%2C+Super+stable+trimer/pm36460696-319-5-13
Average 95 stars, based on 1 article reviews
sars cov 2 spike protein - by Bioz Stars, 2026-09
95/100 stars
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recombinant mouse protein c  (R&D Systems)
94
R&D Systems recombinant mouse protein c
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Recombinant Mouse Protein C, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/Recombinant+Mouse+Protein+S%2FPROS1%2C+CF/bio_rxiv__2021__01__15__426784-190-0-10
Average 94 stars, based on 1 article reviews
recombinant mouse protein c - by Bioz Stars, 2026-09
94/100 stars
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cathepsin s  (R&D Systems)
93
R&D Systems cathepsin s
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Cathepsin S, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/Recombinant+Human+Cathepsin+S+Protein%2C+CF/pm40469052-47-29-31
Average 93 stars, based on 1 article reviews
cathepsin s - by Bioz Stars, 2026-09
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human liver fbpase 1 cdna  (OriGene)
90
OriGene human liver fbpase 1 cdna
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Human Liver Fbpase 1 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/FBP1+(1-338%2C+His-tag)+Human+Protein/pmc05108348-114-0-9
Average 90 stars, based on 1 article reviews
human liver fbpase 1 cdna - by Bioz Stars, 2026-09
90/100 stars
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dc protein assay reagents  (Bio-Rad)
96
Bio-Rad dc protein assay reagents
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Dc Protein Assay Reagents, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/Protein+Assay+Reagent+S/pm25889549-94-7-6
Average 96 stars, based on 1 article reviews
dc protein assay reagents - by Bioz Stars, 2026-09
96/100 stars
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mouse anti s100β antibody  (Proteintech)
96
Proteintech mouse anti s100β antibody
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Mouse Anti S100β Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/S100+beta+Antibody/pm41693354-250-27-31
Average 96 stars, based on 1 article reviews
mouse anti s100β antibody - by Bioz Stars, 2026-09
96/100 stars
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ero1  (Proteintech)
93
Proteintech ero1
FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of <t>GFP-conjugated</t> WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.
Ero1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/ERO1L+Antibody/pm41820988-97-71-73
Average 93 stars, based on 1 article reviews
ero1 - by Bioz Stars, 2026-09
93/100 stars
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anti rad21 rabbit monoclonal antibody  (Proteintech)
93
Proteintech anti rad21 rabbit monoclonal antibody
( A ) GO enrichment analysis of 1650 targeted mRNAs. ( B ) KEGG pathway enrichment analysis of 1650 targeted mRNAs . ( C ) The ceRNA network map of MIF-AS1. ( D ) Analysis of the correlation between MIF-AS1 expression and <t>RAD21</t> expression in NSCLC tumors from the TCGA database (Pearson correlation analysis). ( E ) Knockdown of MIF-AS1 by siRNA decreases RAD21 expression in NSCLC PC9 cells. PC9 cells were transfected with control (si-NC) and MIF-AS1 siRNAs (si-MIF-AS1-1), and the mRNA and protein levels of RAD21 were determined by qPCR and western blot (Student’s t test). ( F ) KM survival analysis of RAD21 in the TCGA dataset. NSCLC patients were divided into low-expression and high-expression groups based on the optimal cut-off point.
Anti Rad21 Rabbit Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/RAD21+Antibody/pmc12966315-98-28-41
Average 93 stars, based on 1 article reviews
anti rad21 rabbit monoclonal antibody - by Bioz Stars, 2026-09
93/100 stars
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anti rabbit vitronectin  (Proteintech)
93
Proteintech anti rabbit vitronectin
Proteomic analysis of FF samples revealed extracellular matrix (ECM) protein - <t>vitronectin</t> as the component responsible for FTE adhesion and spreading A. Experimental workflow of proteomics experiment. B. Venn diagram showing 14 common proteins identified between young and aged FF samples from proteomics analysis. Common proteins are listed with vitronectin (highlighted in red). C. Representative immunoblot for vitronectin expression in 3 young (Y1–Y3) and 3 aged (A1-A3) FF samples. FF samples (5 μg) and recombinant vitronectin protein (0.01 μg, 0.1 μg, 1 μg) diluted with lysis buffer were used for immunoblotting. Arrow represents the band analyzed for vitronectin. Ponceau staining was used for loading control.
Anti Rabbit Vitronectin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+protein/Vitronectin+Antibody/pmc10945186-3-0-3
Average 93 stars, based on 1 article reviews
anti rabbit vitronectin - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of GFP-conjugated WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.

Journal: British journal of pharmacology

Article Title: Veklury® (remdesivir) formulations inhibit initial membrane-coupled events of SARS-CoV-2 infection due to their sulfobutylether-β-cyclodextrin content.

doi: 10.1111/bph.16063

Figure Lengend Snippet: FIGURE 2 Methyl-β cyclodextrin (MβCD)-induced reductions in ACE2 binding of Wuhan-Hu-1 strain of SARS-CoV-2 (WT) and Delta SARS- CoV-2 spike receptor-binding domains (RBDs) negatively correlate with the applied RBD concentrations. HEK/ACE2 + TMPRSS2 cells were treated with different concentrations of MβCD ranging between 0.25 and 10 mM. Fluorescence intensities of cells were measured using flow cytometry for 5 min continuously starting immediately after the addition of GFP-conjugated WT (a) or Delta (b) SARS-CoV-2 spike RBDs applied at various concentrations between 0.1 and 5 μgml1, or 0.05 and 2.5 μgml1, respectively. Moving averages of time-correlated fluorescence intensities of at least 50,000 cells of normal morphology were determined with a window size of 10 s and subsequently normalized to the average of intensities obtained in the last time window of untreated control samples. Mean ± SD values were calculated from data of six independent samples per treatment condition and plotted as a function of time (starting 20 s after RBD addition). The extents of inhibition of WT (a) and Delta (b) RBD binding at the end of the measurement time in response to various MβCD concentrations were calculated and plotted as mean ± SD as a function of the applied RBD concentration in the right panels. Asterisks indicate significant differences of samples treated with the lowest versus highest RBD concentrations at each applied CD concentration (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S2.

Article Snippet: When using Omicron spike trimer conjugated with Alexa Fluor 488 (R&D Systems; AFG11060), experiments were repeated after SBECD, Veklury® P or Veklury® S treatments in HEK/ACE2 + TMPRSS2 and Calu-3 cells using the trimer at 0.5 and 2.5 μg ml 1, respectively.

Techniques: Binding Assay, Fluorescence, Flow Cytometry, Control, Inhibition, Concentration Assay

FIGURE 6 Veklury® (remdesivir) formulations effectively decrease ACE2 binding of Omicron SARS-CoV-2 spike receptor-binding domain (RBD) and the cellular uptake of Omicron SARS-CoV-2 spike trimer due to their SBECD content. (a) HEK/ACE2 + TMPRSS2 (left panel) or Calu-3 cells (right panel) were treated with SBECD, Veklury® P (powder) or Veklury® S (solution) at CD concentrations of 1, 5 and 10 mM. Then, HEK/ ACE2 + TMPRSS2 cells were incubated in the presence of 0.2-μgml1 Omicron SARS-CoV-2 spike RBD–GFP, whereas Calu-3 cells were labelled with 1-μgml1 Omicron RBD–GFP for 4 min. The emitted fluorescence intensities of individual cells were subsequently measured using flow cytometry, and the mean intensity was calculated from data of at least 10,000 cells of normal morphology per sample. The average values of six independent measurements (±SD) were calculated, normalized to the mean value determined in untreated control samples and plotted as a function of the applied concentrations of CD. Asterisks indicate significant differences of samples treated with the highest applied concentrations compared to the control samples (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S6. (b) HEK/ACE2 + TMPRSS2 (left panel) or Calu-3 cells (right panel) treated with SBECD, Veklury® P or Veklury® S at CD concentrations of 1 and 5 mM for 1 h were subsequently incubated for 4 h in the presence of Omicron SARS-CoV-2 spike trimers conjugated with Alexa Fluor 488. The average fluorescence intensity values emitted by Alexa Fluor 488-labelled trimers were calculated using data of intracellular pixels identified using the membrane marker N-[3-(40-dihexylamino-3-hydroxy-flavonyl-6-oxy)-propyl]N,N-dimethyl-N-(3-sulfopropyl)-ammonium inner salt (F66) as described in Figure 5 for individual cells, which were subsequently normalized to the median value determined in untreated control samples. The number of cells obtained from five independent experiments and involved in the analysis was from left to right: 382, 348, 373, 396, 388, 402 and 393 for Omicron trimer uptake in HEK/ACE2 + TMPRSS2 and 308, 250, 256, 223, 222, 232 and 296 for Omicron trimer uptake in Calu-3. Data points indicated in the figure were obtained from individual cells and plotted along with median values with quartiles. Grey shaded areas show ranges between 0.5 and 1. Asterisks indicate significant differences compared to the control samples (*P < 0.05; one-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S6.

Journal: British journal of pharmacology

Article Title: Veklury® (remdesivir) formulations inhibit initial membrane-coupled events of SARS-CoV-2 infection due to their sulfobutylether-β-cyclodextrin content.

doi: 10.1111/bph.16063

Figure Lengend Snippet: FIGURE 6 Veklury® (remdesivir) formulations effectively decrease ACE2 binding of Omicron SARS-CoV-2 spike receptor-binding domain (RBD) and the cellular uptake of Omicron SARS-CoV-2 spike trimer due to their SBECD content. (a) HEK/ACE2 + TMPRSS2 (left panel) or Calu-3 cells (right panel) were treated with SBECD, Veklury® P (powder) or Veklury® S (solution) at CD concentrations of 1, 5 and 10 mM. Then, HEK/ ACE2 + TMPRSS2 cells were incubated in the presence of 0.2-μgml1 Omicron SARS-CoV-2 spike RBD–GFP, whereas Calu-3 cells were labelled with 1-μgml1 Omicron RBD–GFP for 4 min. The emitted fluorescence intensities of individual cells were subsequently measured using flow cytometry, and the mean intensity was calculated from data of at least 10,000 cells of normal morphology per sample. The average values of six independent measurements (±SD) were calculated, normalized to the mean value determined in untreated control samples and plotted as a function of the applied concentrations of CD. Asterisks indicate significant differences of samples treated with the highest applied concentrations compared to the control samples (*P < 0.05; two-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S6. (b) HEK/ACE2 + TMPRSS2 (left panel) or Calu-3 cells (right panel) treated with SBECD, Veklury® P or Veklury® S at CD concentrations of 1 and 5 mM for 1 h were subsequently incubated for 4 h in the presence of Omicron SARS-CoV-2 spike trimers conjugated with Alexa Fluor 488. The average fluorescence intensity values emitted by Alexa Fluor 488-labelled trimers were calculated using data of intracellular pixels identified using the membrane marker N-[3-(40-dihexylamino-3-hydroxy-flavonyl-6-oxy)-propyl]N,N-dimethyl-N-(3-sulfopropyl)-ammonium inner salt (F66) as described in Figure 5 for individual cells, which were subsequently normalized to the median value determined in untreated control samples. The number of cells obtained from five independent experiments and involved in the analysis was from left to right: 382, 348, 373, 396, 388, 402 and 393 for Omicron trimer uptake in HEK/ACE2 + TMPRSS2 and 308, 250, 256, 223, 222, 232 and 296 for Omicron trimer uptake in Calu-3. Data points indicated in the figure were obtained from individual cells and plotted along with median values with quartiles. Grey shaded areas show ranges between 0.5 and 1. Asterisks indicate significant differences compared to the control samples (*P < 0.05; one-way ANOVA followed by Tukey's HSD test). See details of statistical analysis in Table S6.

Article Snippet: When using Omicron spike trimer conjugated with Alexa Fluor 488 (R&D Systems; AFG11060), experiments were repeated after SBECD, Veklury® P or Veklury® S treatments in HEK/ACE2 + TMPRSS2 and Calu-3 cells using the trimer at 0.5 and 2.5 μg ml 1, respectively.

Techniques: Binding Assay, Incubation, Fluorescence, Flow Cytometry, Control, Membrane, Marker

( A ) GO enrichment analysis of 1650 targeted mRNAs. ( B ) KEGG pathway enrichment analysis of 1650 targeted mRNAs . ( C ) The ceRNA network map of MIF-AS1. ( D ) Analysis of the correlation between MIF-AS1 expression and RAD21 expression in NSCLC tumors from the TCGA database (Pearson correlation analysis). ( E ) Knockdown of MIF-AS1 by siRNA decreases RAD21 expression in NSCLC PC9 cells. PC9 cells were transfected with control (si-NC) and MIF-AS1 siRNAs (si-MIF-AS1-1), and the mRNA and protein levels of RAD21 were determined by qPCR and western blot (Student’s t test). ( F ) KM survival analysis of RAD21 in the TCGA dataset. NSCLC patients were divided into low-expression and high-expression groups based on the optimal cut-off point.

Journal: Scientific Reports

Article Title: A lncRNA and radiomics-based model for predicting the response of non-small cell lung cancer to chemo- and radio-therapy

doi: 10.1038/s41598-026-39560-x

Figure Lengend Snippet: ( A ) GO enrichment analysis of 1650 targeted mRNAs. ( B ) KEGG pathway enrichment analysis of 1650 targeted mRNAs . ( C ) The ceRNA network map of MIF-AS1. ( D ) Analysis of the correlation between MIF-AS1 expression and RAD21 expression in NSCLC tumors from the TCGA database (Pearson correlation analysis). ( E ) Knockdown of MIF-AS1 by siRNA decreases RAD21 expression in NSCLC PC9 cells. PC9 cells were transfected with control (si-NC) and MIF-AS1 siRNAs (si-MIF-AS1-1), and the mRNA and protein levels of RAD21 were determined by qPCR and western blot (Student’s t test). ( F ) KM survival analysis of RAD21 in the TCGA dataset. NSCLC patients were divided into low-expression and high-expression groups based on the optimal cut-off point.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST for 2 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies: anti-Rad21 rabbit monoclonal antibody (Absin, Shanghai, China; 1:1000) and anti-β-Actin mouse monoclonal antibody (Proteintech, Wuhan, China; 1:5000).

Techniques: Expressing, Knockdown, Transfection, Control, Western Blot

Proteomic analysis of FF samples revealed extracellular matrix (ECM) protein - vitronectin as the component responsible for FTE adhesion and spreading A. Experimental workflow of proteomics experiment. B. Venn diagram showing 14 common proteins identified between young and aged FF samples from proteomics analysis. Common proteins are listed with vitronectin (highlighted in red). C. Representative immunoblot for vitronectin expression in 3 young (Y1–Y3) and 3 aged (A1-A3) FF samples. FF samples (5 μg) and recombinant vitronectin protein (0.01 μg, 0.1 μg, 1 μg) diluted with lysis buffer were used for immunoblotting. Arrow represents the band analyzed for vitronectin. Ponceau staining was used for loading control.

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Proteomic analysis of FF samples revealed extracellular matrix (ECM) protein - vitronectin as the component responsible for FTE adhesion and spreading A. Experimental workflow of proteomics experiment. B. Venn diagram showing 14 common proteins identified between young and aged FF samples from proteomics analysis. Common proteins are listed with vitronectin (highlighted in red). C. Representative immunoblot for vitronectin expression in 3 young (Y1–Y3) and 3 aged (A1-A3) FF samples. FF samples (5 μg) and recombinant vitronectin protein (0.01 μg, 0.1 μg, 1 μg) diluted with lysis buffer were used for immunoblotting. Arrow represents the band analyzed for vitronectin. Ponceau staining was used for loading control.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: Western Blot, Expressing, Recombinant, Lysis, Staining, Control

Vitronectin in FF aids in FTE adhesion and spreading A. Representative brightfield images of FT190 cells seeded on ULA plates coated with FF sample (400 μl) and recombinant vitronectin protein (1 μg/well) for 4 h. Wells were washed with 1X PBS and FT190 cells were seeded on the coated plates. Images were acquired after 24 h. Scale bar = 200 μm. B. Cell proliferation was measured using an SRB assay to measure cell viability of FT190 cells on FF and vitronectin coated ULA plates. C. Representative Z stack images acquired by confocal microscopy of FT190 spheroids (labelled with Cell tracker 594) on NOF151 cells (labelled with Cell tracker 488) with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 200 μm. D. A maximum intensity projection re-construction of a three-dimensional FTE spheroid (labelled with Cell tracker 594) optical data stack over the surface of NOF151 cells (labelled with Cell tracker 488) treated with FF samples (Y1, A1) and vitronectin (1 μg). Spheroids were imaged with a 10× objective and re-constructed using the Imaris software. E. Representative images acquired by confocal microscopy showing a side and top projection of the FT190 spheroids on NOF151 cells with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 100 μm. F. Spheroid area (with and without FF/vitronectin) was determined by quantification of red fluorescent intensity using a polygon area measurement tool of the Imaris software.

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Vitronectin in FF aids in FTE adhesion and spreading A. Representative brightfield images of FT190 cells seeded on ULA plates coated with FF sample (400 μl) and recombinant vitronectin protein (1 μg/well) for 4 h. Wells were washed with 1X PBS and FT190 cells were seeded on the coated plates. Images were acquired after 24 h. Scale bar = 200 μm. B. Cell proliferation was measured using an SRB assay to measure cell viability of FT190 cells on FF and vitronectin coated ULA plates. C. Representative Z stack images acquired by confocal microscopy of FT190 spheroids (labelled with Cell tracker 594) on NOF151 cells (labelled with Cell tracker 488) with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 200 μm. D. A maximum intensity projection re-construction of a three-dimensional FTE spheroid (labelled with Cell tracker 594) optical data stack over the surface of NOF151 cells (labelled with Cell tracker 488) treated with FF samples (Y1, A1) and vitronectin (1 μg). Spheroids were imaged with a 10× objective and re-constructed using the Imaris software. E. Representative images acquired by confocal microscopy showing a side and top projection of the FT190 spheroids on NOF151 cells with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 100 μm. F. Spheroid area (with and without FF/vitronectin) was determined by quantification of red fluorescent intensity using a polygon area measurement tool of the Imaris software.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: Recombinant, Sulforhodamine B Assay, Confocal Microscopy, Construct, Software

Primary antibodies.

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Primary antibodies.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: